Summary:

Purpose

Our Quick Step Human visfatin ELISA Kit is to assay visfatin levels in Human serum, plasma, culture media or any biological fluid.

 

Principle

This ELISA kit uses Sandwich-ELISA as the method. The Microelisa stripplate provided in this kit has been pre-coated with an antibody specific to visfatin. Standards or samples are added to the appropriate Microelisa stripplate wells and combined to the specific antibody. Then a Horseradish Peroxidase (HRP)-conjugated antibody specific for visfatin is added to each Microelisa stripplate well and incubated. Free components are washed away. The TMB substrate solution is added to each well. Only those wells that contain visfatin and HRP conjugated visfatin antibody will appear blue in color and then turn yellow after the addition of the stop solution. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm. The OD value is proportional to the concentration of visfatin. You can calculate the concentration of visfatin in the samples by comparing the OD of the samples to the standard curve.

Typical Data

 The standard curve of QS1825Hu is provided for demonstration only. A standard curve should be generated for each set of samples assayed.

 

Standard	Concentration	OD Value	Average OD Value
Blank Well	0.00ng/ml	0.053	0.0385
		0.052
S1	6.00 ng/ml	1.951	1.9820
		1.937
S2	4.00 ng/ml	1.311	1.3780
		1.319
S3	2.00 ng/ml	0.718	0.7290
		0.726
S4	1.00 ng/ml	0.391	0.3795
		0.387
S5	0.50 ng/ml	0.213	0.1800
		0.205

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