Summary:

Purpose

Our Quick Step Human intestinal Fatty Acid Binding Protein (iFABP/FABP2)ELISA Kit is to assay iFABP/FABP2 levels in Human serum, plasma, culture media or any biological fluid.

 

Principle

This ELISA kit uses Sandwich-ELISA as the method. The Microelisa stripplate provided in this kit has been pre-coated with an antibody specific to iFABP/FABP2. Standards or samples are added to the appropriate Microelisa stripplate wells and combined to the specific antibody. Then a Horseradish Peroxidase (HRP)-conjugated antibody specific for iFABP/FABP2 is added to each Microelisa stripplate well and incubated. Free components are washed away. The TMB substrate solution is added to each well. Only those wells that contain iFABP/FABP2 and HRP conjugated iFABP/FABP2 antibody will appear blue in color and then turn yellow after the addition of the stop solution. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm. The OD value is proportional to the concentration of iFABP/FABP2. You can calculate the concentration of iFABP/FABP2 in the samples by comparing the OD of the samples to the standard curve.

Typical Data

 The standard curve of CQS1010Hu is provided for demonstration only. A standard curve should be generated for each set of samples assayed. 

 

Standard	Concentration	OD Value	Average OD Value
Blank Well	0ng/ml	0.056	0.0540
		0.052
S1	30ng/ml	1.995	1.9890
		1.983
S2	20ng/ml	1.425	1.4195
		1.414
S3	10ng/ml	0.752	0.7455
		0.739
S4	5ng/ml	0.415	0.4085
		0.402
S5	2.5ng/ml	0.201	0.1975
		0.194

 

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