Summary:

Purpose

Our Quick Step Human RAC-Alpha Serine/Threonine-Protein Kinase (AKT1)ELISA Kit is to assay AKT1 levels in Human serum, plasma, culture media or any biological fluid.

 

Principle

This ELISA kit uses Sandwich-ELISA as the method. The Microelisa stripplate provided in this kit has been pre-coated with an antibody specific to AKT1. Standards or samples are added to the appropriate Microelisa stripplate wells and combined to the specific antibody. Then a Horseradish Peroxidase (HRP)-conjugated antibody specific for AKT1 is added to each Microelisa stripplate well and incubated. Free components are washed away. The TMB substrate solution is added to each well. Only those wells that contain AKT1 and HRP conjugated AKT1 antibody will appear blue in color and then turn yellow after the addition of the stop solution. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm. The OD value is proportional to the concentration of AKT1. You can calculate the concentration of AKT1 in the samples by comparing the OD of the samples to the standard curve.

Typical Data

 The standard curve of QS4488Hu is provided for demonstration only. A standard curve should be generated for each set of samples assayed. 

Standard	Concentration	OD Value	Average OD Value
Blank Well	0pg/ml	0.049	0.0440
		0.039
S1	1800pg/ml	1.956	1.9530
		1.950
S2	1200pg/ml	1.389	1.3910
		1.393
S3	600pg/ml	0.709	0.7135
		0.718
S4	300pg/ml	0.390	0.3875
		0.385
S5	150pg/ml	0.195	0.2000
		0.205

 

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