Summary:

Purpose

Our Quick Step Human Sirtuin 4 (SIRT4)ELISA Kit is to assay SIRT4 levels in Human serum, plasma, culture media or any biological fluid.

 

Principle

This ELISA kit uses Sandwich-ELISA as the method. The Microelisa stripplate provided in this kit has been pre-coated with an antibody specific to SIRT4. Standards or samples are added to the appropriate Microelisa stripplate wells and combined to the specific antibody. Then a Horseradish Peroxidase (HRP)-conjugated antibody specific for SIRT4 is added to each Microelisa stripplate well and incubated. Free components are washed away. The TMB substrate solution is added to each well. Only those wells that contain SIRT4 and HRP conjugated SIRT4 antibody will appear blue in color and then turn yellow after the addition of the stop solution. The optical density (OD) is measured spectrophotomeSIRT4ally at a wavelength of 450 nm. The OD value is proportional to the concentration of SIRT4. You can calculate the concentration of SIRT4 in the samples by comparing the OD of the samples to the standard curve.

Typical Data

 The standard curve of QS4543Hu is provided for demonstration only. A standard curve should be generated for each set of samples assayed. 

Standard	Concentration	OD Value	Average OD Value
Blank Well	0pg/ml	0.052	0.0460
		0.040
S1	3600pg/ml	1.912	1.9075
		1.903
S2	2400pg/ml	1.388	1.3840
		1.380
S3	1200pg/ml	0.708	0.7100
		0.712
S4	600pg/ml	0.403	0.4000
		0.397
S5	300pg/ml	0.189	0.1960
		0.203

 

 

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