Summary:

Purpose

Our Quick Step Human Sirtuin 2 (SIRT2)ELISA Kit is to assay SIRT2 levels in Human serum, plasma, culture media or any biological fluid.

 

Principle

This ELISA kit uses Sandwich-ELISA as the method. The Microelisa stripplate provided in this kit has been pre-coated with an antibody specific to SIRT2. Standards or samples are added to the appropriate Microelisa stripplate wells and combined to the specific antibody. Then a Horseradish Peroxidase (HRP)-conjugated antibody specific for SIRT2 is added to each Microelisa stripplate well and incubated. Free components are washed away. The TMB substrate solution is added to each well. Only those wells that contain SIRT2 and HRP conjugated SIRT2 antibody will appear blue in color and then turn yellow after the addition of the stop solution. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm. The OD value is proportional to the concentration of SIRT2. You can calculate the concentration of SIRT2 in the samples by comparing the OD of the samples to the standard curve.

Typical Data

 The standard curve of QS4542Hu is provided for demonstration only. A standard curve should be generated for each set of samples assayed. 

 

Standard	Concentration	OD Value	Average OD Value
Blank Well	0ng/ml	0.048	0.0465
		0.045
S1	18ng/ml	1.973	1.9760
		1.979
S2	12ng/ml	1.363	1.3675
		1.372
S3	6ng/ml	0.725	0.7220
		0.719
S4	3ng/ml	0.388	0.3860
		0.384
S5	1.5ng/ml	0.187	0.1895
		0.192

 

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