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Summary:
Purpose
Our Quick Step Mouse Glutathione Peroxidase 1 (GPx-1)ELISA Kit is to assay GPx-1 levels in Mouse serum, plasma, culture media or any biological fluid.
Principle
This ELISA kit uses Sandwich-ELISA as the method. The Microelisa stripplate provided in this kit has been pre-coated with an antibody specific to GPx-1. Standards or samples are added to the appropriate Microelisa stripplate wells and combined to the specific antibody. Then a Horseradish Peroxidase (HRP)-conjugated antibody specific for GPx-1 is added to each Microelisa stripplate well and incubated. Free components are washed away. The TMB substrate solution is added to each well. Only those wells that contain GPx-1 and HRP conjugated GPx-1 antibody will appear blue in color and then turn yellow after the addition of the stop solution. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm. The OD value is proportional to the concentration of GPx-1. You can calculate the concentration of GPx-1 in the samples by comparing the OD of the samples to the standard curve.
Typical Data
The standard curve of QS1062Mo is provided for demonstration only. A standard curve should be generated for each set of samples assayed.
Standard
Concentration
OD Value
Average OD Value
Blank Well
0
0.047
0.0440
0.041
S1
36ng/ml
1.967
1.9610
1.955
S2
24ng/ml
1.315
1.3200
1.325
S3
12ng/ml
0.685
0.6930
0.701
S4
6ng/ml
0.391
0.3880
0.385
S5
3ng/ml
0.183
0.1875
0.192
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