Summary:

 

Purpose

Our Quick Step Mouse Glutathione Peroxidase 1 (GPx-1)ELISA Kit is to assay GPx-1 levels in Mouse serum, plasma, culture media or any biological fluid.

 

Principle

This ELISA kit uses Sandwich-ELISA as the method. The Microelisa stripplate provided in this kit has been pre-coated with an antibody specific to GPx-1. Standards or samples are added to the appropriate Microelisa stripplate wells and combined to the specific antibody. Then a Horseradish Peroxidase (HRP)-conjugated antibody specific for GPx-1 is added to each Microelisa stripplate well and incubated. Free components are washed away. The TMB substrate solution is added to each well. Only those wells that contain GPx-1 and HRP conjugated GPx-1 antibody will appear blue in color and then turn yellow after the addition of the stop solution. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm. The OD value is proportional to the concentration of GPx-1. You can calculate the concentration of GPx-1 in the samples by comparing the OD of the samples to the standard curve.

Typical Data

 The standard curve of QS1062Mo is provided for demonstration only. A standard curve should be generated for each set of samples assayed. 

 

Standard	Concentration	OD Value	Average OD Value
Blank Well	0	0.047	0.0440
		0.041
S1	36ng/ml	1.967	1.9610
		1.955
S2	24ng/ml	1.315	1.3200
		1.325
S3	12ng/ml	0.685	0.6930
		0.701
S4	6ng/ml	0.391	0.3880
		0.385
S5	3ng/ml	0.183	0.1875
		0.192

 

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