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Quick Step Mouse Glutathione Peroxidase 1 (GPx-1)ELISA Kit
Quick Step Mouse Glutathione Peroxidase 1 (GPx-1)ELISA Kit
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  • NO.:QS1062Mo
    Sensitivity:0.05ng/ml
    Assay range:0.48ng/ml-40ng/ml
    Standard:54.0ng/ml
    Sample Volume:10ul
    Wavelength:450nm
    Assay Time:60 minutes
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Summary:

 

Purpose

Our Quick Step Mouse Glutathione Peroxidase 1 (GPx-1)ELISA Kit is to assay GPx-1 levels in Mouse serum, plasma, culture media or any biological fluid.

 

Principle

This ELISA kit uses Sandwich-ELISA as the method. The Microelisa stripplate provided in this kit has been pre-coated with an antibody specific to GPx-1. Standards or samples are added to the appropriate Microelisa stripplate wells and combined to the specific antibody. Then a Horseradish Peroxidase (HRP)-conjugated antibody specific for GPx-1 is added to each Microelisa stripplate well and incubated. Free components are washed away. The TMB substrate solution is added to each well. Only those wells that contain GPx-1 and HRP conjugated GPx-1 antibody will appear blue in color and then turn yellow after the addition of the stop solution. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm. The OD value is proportional to the concentration of GPx-1. You can calculate the concentration of GPx-1 in the samples by comparing the OD of the samples to the standard curve.

Typical Data

 The standard curve of QS1062Mo is provided for demonstration only. A standard curve should be generated for each set of samples assayed. 

 

Standard

Concentration

OD Value

Average OD Value

Blank Well

0

0.047

0.0440

0.041

S1

36ng/ml

1.967

1.9610

1.955

S2

24ng/ml

1.315

1.3200

1.325

S3

12ng/ml

0.685

0.6930

0.701

S4

6ng/ml

0.391

0.3880

0.385

S5

3ng/ml

0.183

0.1875

0.192

 

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