Summary:

 

Purpose

Our Quick Step Mouse 78 kDa Glucose-Regulated Protein (HSPA5)ELISA Kit is to assay HSPA5 levels in Mouse serum, plasma, culture media or any biological fluid.

 

Principle

This ELISA kit uses Sandwich-ELISA as the method. The Microelisa stripplate provided in this kit has been pre-coated with an antibody specific to HSPA5. Standards or samples are added to the appropriate Microelisa stripplate wells and combined to the specific antibody. Then a Horseradish Peroxidase (HRP)-conjugated antibody specific for HSPA5 is added to each Microelisa stripplate well and incubated. Free components are washed away. The TMB substrate solution is added to each well. Only those wells that contain HSPA5 and HRP conjugated HSPA5 antibody will appear blue in color and then turn yellow after the addition of the stop solution. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm. The OD value is proportional to the concentration of HSPA5. You can calculate the concentration of HSPA5 in the samples by comparing the OD of the samples to the standard curve.

Typical Data

 The standard curve of QS1063Mo is provided for demonstration only. A standard curve should be generated for each set of samples assayed. 

Standard	Concentration	OD Value	Average OD Value
Blank Well	0pg/ml	0.049	0.0440
		0.039
S1	1800pg/ml	1.956	1.9530
		1.950
S2	1200pg/ml	1.389	1.3910
		1.393
S3	600pg/ml	0.709	0.7135
		0.718
S4	300pg/ml	0.390	0.3875
		0.385
S5	150pg/ml	0.195	0.2000
		0.205

 

 

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