Aliases:	ACAT1; Acetyl-CoA Acetyltransferase 1; THIL; ACAT; Acetyl-CoA Acetyltransferase, Mitochondrial; Acetyl-Coenzyme A Acetyltransferase 1; Acetoacetyl Coenzyme A Thiolase; Acetoacetyl-CoA Thiolase; EC 2.3.1.9; MAT; T2; Mitochondrial Acetoacetyl-CoA Thiolase; Testicular Tissue Protein Li 198; EC 2.3.1
Background:	UniProt Entry: P24752;NCBI Gene Entry: 38
Application Information	Molecular Weight: Predicted, 45 kDa, observed, 40 kDa Clonality: Rabbit monoclonal antibody Clone ID: 23GB1255 Species Reactivity: Human, Mouse, Rat Applications Tested: Western Blotting (WB), Flow Cytometry (FCM), Immunocytochemistry (IC)
Immunogen	A synthesized peptide derived from human ACAT1
Isotype	Rabbit IgG
Storage Buffer	Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Storage	Store at -20 °C for one year.
Recommended Dilutions	Western Blotting (WB): 1:1,000-1:5,000 Flow Cytometry (FCM): 1:2,000 Immunocytochemistry (IC): 1:1,000
Protocols	For general and specific antibody protocols please visit our website. Read all instructions before using this product.

Flow cytometric analysis of Acetyl-CoA acetyltransferase 1 expression in HepG2 cells using Acetyl-CoA acetyltransferase 1 antibody 1:2,000. Green, isotype control; red, Acetyl-CoA acetyltransferase 1.

Immunocytochemical staining of HT-1080 cells with Acetyl-CoA acetyltransferase 1 antibody 1:1,000 . Nuclei were stained blue with DAPI;Acetyl-CoA acetyltransferase 1 with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar: 20 μm.

Western blotting analysis using anti-Acetyl-CoA acetyltransferase 1 antibody 1:5,000 and HRP-conjugated goat anti-rabbit secondary antibody 1:20,000 respectively.

Western blotting analysis using anti-Acetyl-CoA acetyltransferase 1 antibody 1:5,000 and HRP-conjugated goat anti-rabbit secondary antibody 1:20,000 respectively.

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