Aliases:	ADGRE5; Adhesion G Protein-Coupled Receptor E5; Leukocyte Antigen CD97; TM7LN1; CD97; Seven-Transmembrane, Heterodimeric Receptor Associated With Inflammation; Seven Transmembrane Helix Receptor; Seven-Span Transmembrane Protein; CD97 Molecule; CD97 Antigen
Background:	UniProt Entry: P48960;NCBI Gene Entry: 976
Application Information	Molecular Weight: Predicted, 92 kDa, observed, 40 kDa Clonality: Rabbit monoclonal antibody Clone ID: 23GB3740 Species Reactivity: Human, Mouse, Rat Applications Tested: Western Blotting (WB), Flow Cytometry (FCM), Immunocytochemistry (IC)
Immunogen	A synthesized peptide derived from human CD97
Isotype	Rabbit IgG
Storage Buffer	Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Storage	Store at -20 °C for one year.
Recommended Dilutions	Western Blotting (WB): 1:1,000-1:5,000 Flow Cytometry (FCM): 1:2,000 Immunocytochemistry (IC): 1:1,000
Protocols	For general and specific antibody protocols please visit our website. Read all instructions before using this product.

Flow cytometric analysis of CD97 expression in HepG2 cells using CD97 antibody 1:2,000. Green, isotype control; red, CD97.

Immunocytochemical staining of HepG2 cells with CD97 antibody 1:1,000. Nuclei were stained blue with DAPI; CD97 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar: 20 μm.

Western blotting analysis using anti-CD97 antibody 1:5,000 and HRP-conjugated goat anti-rabbit secondary antibody 1:20,000 respectively.

Western blotting analysis using anti-CD97 antibody 1:5,000 and HRP-conjugated goat anti-rabbit secondary antibody 1:20,000 respectively.

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