Summary:

 

Purpose

Our Quick Step Sheep Neopterin (NEOP)ELISA Kit is to assay NEOP levels in Sheep serum, plasma, culture media or any biological fluid.

 

Principle

This ELISA kit uses Sandwich-ELISA as the method. The Microelisa stripplate provided in this kit has been pre-coated with an antibody specific to NEOP. Standards or samples are added to the appropriate Microelisa stripplate wells and combined to the specific antibody. Then a Horseradish Peroxidase (HRP)-conjugated antibody specific for NEOP is added to each Microelisa stripplate well and incubated. Free components are washed away. The TMB substrate solution is added to each well. Only those wells that contain NEOP and HRP conjugated NEOP antibody will appear blue in color and then turn yellow after the addition of the stop solution. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm. The OD value is proportional to the concentration of NEOP. You can calculate the concentration of NEOP in the samples by comparing the OD of the samples to the standard curve.

Typical Data

 The standard curve of QS00169Sp is provided for demonstration only. A standard curve should be generated for each set of samples assayed. 

Standard	Concentration	OD Value	Average OD Value
Blank Well	0pmol/ml	0.043	0.0405
		0.038
S1	90pmol/ml	1.954	1.9615
		1.969
S2	60pmol/ml	1.318	1.3305
		1.343
S3	30pmol/ml	0.683	0.6910
		0.699
S4	15pmol/ml	0.391	0.3865
		0.382
S5	7.5pmol/ml	0.194	0.1910
		0.188

 

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