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Quick Step Sheep Neopterin (NEOP)ELISA Kit
Quick Step Sheep Neopterin (NEOP)ELISA Kit
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Regular members: $422.4
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  • NO.:QS00179Sp
    Sensitivity:0.2pmol/ml
    Assay range:1.1pmol/ml-100pmol/ml
    Standard:135pmol/ml
    Sample Volume:10ul
    Wavelength:450nm
    Assay Time:60 minutes
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Details

Summary:

 

Purpose

Our Quick Step Sheep Neopterin (NEOP)ELISA Kit is to assay NEOP levels in Sheep serum, plasma, culture media or any biological fluid.

 

Principle

This ELISA kit uses Sandwich-ELISA as the method. The Microelisa stripplate provided in this kit has been pre-coated with an antibody specific to NEOP. Standards or samples are added to the appropriate Microelisa stripplate wells and combined to the specific antibody. Then a Horseradish Peroxidase (HRP)-conjugated antibody specific for NEOP is added to each Microelisa stripplate well and incubated. Free components are washed away. The TMB substrate solution is added to each well. Only those wells that contain NEOP and HRP conjugated NEOP antibody will appear blue in color and then turn yellow after the addition of the stop solution. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm. The OD value is proportional to the concentration of NEOP. You can calculate the concentration of NEOP in the samples by comparing the OD of the samples to the standard curve.

Typical Data

 The standard curve of QS00169Sp is provided for demonstration only. A standard curve should be generated for each set of samples assayed. 

Standard

Concentration

OD Value

Average OD Value

Blank Well

0pmol/ml

0.043

0.0405

0.038

S1

90pmol/ml

1.954

1.9615

1.969

S2

60pmol/ml

1.318

1.3305

1.343

S3

30pmol/ml

0.683

0.6910

0.699

S4

15pmol/ml

0.391

0.3865

0.382

S5

7.5pmol/ml

0.194

0.1910

0.188

 

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