Summary:

 

Purpose

Our Quick Step Cat Homocysteine (HCY)ELISA Kit is to assay HCY levels in Cat serum, plasma, culture media or any biological fluid.

 

Principle

This ELISA kit uses Sandwich-ELISA as the method. The Microelisa stripplate provided in this kit has been pre-coated with an antibody specific to HCY. Standards or samples are added to the appropriate Microelisa stripplate wells and combined to the specific antibody. Then a Horseradish Peroxidase (HRP)-conjugated antibody specific for HCY is added to each Microelisa stripplate well and incubated. Free components are washed away. The TMB substrate solution is added to each well. Only those wells that contain HCY and HRP conjugated HCY antibody will appear blue in color and then turn yellow after the addition of the stop solution. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm. The OD value is proportional to the concentration of HCY. You can calculate the concentration of HCY in the samples by comparing the OD of the samples to the standard curve.

Typical Data

 The standard curve of QS0083Fe is provided for demonstration only. A standard curve should be generated for each set of samples assayed. 

Standard	Concentration	OD Value	Average OD Value
Blank Well	0nmol/ml	0.043	0.0460
		0.049
S1	9nmol/ml	1.933	1.9385
		1.944
S2	6nmol/ml	1.394	1.4015
		1.409
S3	3nmol/ml	0.713	0.7095
		0.706
S4	1.5nmol/ml	0.400	0.3970
		0.394
S5	0.75nmol/ml	0.206	0.2125
		0.219

 

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