Package: 500T/1000T

Storage: MTT Solution stored at -20℃; Formazan Dissolving Solution stored at room temperature or -20℃ condition.

Kit Components

 

Component	500T	1000T
MTT Solution	5mL	5mL×2
Formazan Dissolving Solution	60mL	60mL×2

Product Description

The  kit  is  designed  for  the  spectrophotometric  measurement  of  cell  growth  as  a  function  of mitochondrial  activity in living  cells. Using MTT could measure the activity of living cells via mitochondrial dehydrogenase    activity   with    simple,    accurate    and   reproducible    functions. Dehydrogenases in mitochondria can deoxidize MTT to crystalline purple product Formazan. The crystals  can  be  dissolved  in   specific  solvent.  Use  Microplate  Absorbance  Reader  to  measure absorbance near 490nm wavelength. An increase in cell number results in an increase in the amount ofMTT Formazan formed and an increase in absorbance.

For best  results,  cell  numbers  should  be  determined  during  log  growth  stage.  Each  test  should include a blank containing complete culture medium without cells.

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