Diaminobenzidine (DAB) is the most sensitive and commonly used chromogenic substrate for horseradish peroxidase, and the reaction product  is a brown precipitate insoluble in water, xy lene and alcohols. This product adopts special formula with high sensitivity, low background, stable storage and easy to use. Suitable  for  staining  and  chromogenic  reactions  in  western  blotting, immunohistochemistry and immunocytochemistry, spot blots and biochips, etc.

 

Packing specifications:

Component	200T
DAB concentrate (50X)	250μL
DAB diluent	12.5 mL

Usage method:

1. Prepare 1x DAB color working solution before use: add 20 μL DAB of concentration (50X) to

every 1mL DAB of dilution, mix well and reserve. Need to be ready for current use, limited to the same day of use, long-term preservation is not recommended. Note: Do not use a buffer

containing sodium azide, an HRP inhibitor.

2. After the last washing of the sample, remove the washing solution and add enough DAB color working solution to ensure adequate coverage of the sample; incubate at room

temperature for 3-30 min or longer until the desired depth. Immunoblot experiments should ensure that the sample is in an enough staining solution and can be freely shaken.

3. For immunohistochemistry or in situ hybridization, using 50 μ L of color solution for each sample, this kit can detect a minimum of 200 samples.

4 The DAB staining working solution was removed and the samples were rinsed several times with PBS to abort the chromoreaction.

5. For the cell or tissue samples observed with the microscope, the samples can be

counterstained (optional) and mounted in the aqueous sealing media. Alternatively, the sample may be dehydrated and mounted in organic sealants. For immunoblotting

experiments, samples can be rinsed with water, air dried and then stored at room temperature.

Storage and transportation:

2-8℃ Closed storage away from light, effective for one year. Avoid repeated freezing and thawing.

 

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