Drug Names

Generic Name: Human Anti-Bullous Pemphigoid 180 Antibody (BP180-Ab)ELISA Kit

Purpose

Our Human Anti-Bullous Pemphigoid 180 Antibody (BP180-Ab)ELISA kit is to assay BP180-Ab levels in Human serum, plasma, culture media or any biological fluid.

Principle

This ELISA kit uses Sandwich-ELISA as the method. The Microelisa stripplate provided in this kit has been pre-coated with an antigen specific to BP180-Ab. Standards or samples are added to the appropriate Microelisa stripplate wells and combined to the specific antigen . Then a Horseradish Peroxidase (HRP)-conjugated antigen specific for BP180 is added to each Microelisa stripplate well and incubated. Free components are washed away. The TMB substrate solution is added to each well. Only those wells that contain BP180-Ab and HRP conjugated BP180 antigen will appear blue in color and then turn yellow after the addition of the stop solution. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm. The OD value is proportional to the concentration of BP180-Ab. You can calculate the concentration of BP180-Ab in the samples by comparing the OD of the samples to the standard curve.

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