Drug Names

Generic Name: Human Tumor Necrosis Factor Receptor Superfamily, Member 3B (TNFRSF13B)ELISA Kit

Purpose

Our Human Tumor Necrosis Factor Receptor Superfamily, Member 13B (TNFRSF13B)ELISA kit is to assay TNFRSF13B levels in Human serum, plasma, culture media or any biological fluid.

Principle

 

This ELISA kit uses Sandwich-ELISA as the method. The Microelisa stripplate provided in this kit has been pre-coated with an antibody specific to TNFRSF13B. Standards or samples are added to the appropriate Microelisa stripplate wells and combined to the specific antibody. Then a Horseradish Peroxidase (HRP)-conjugated antibody specific for TNFRSF13B is added to each Microelisa stripplate well and incubated. Free components are washed away. The TMB substrate solution is added to each well. Only those wells that contain TNFRSF13B and HRP conjugated TNFRSF13B antibody will appear blue in color and then turn yellow after the addition of the stop solution. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm. The OD value is proportional to the concentration of TNFRSF13B. You can calculate the concentration of TNFRSF13B in the samples by comparing the OD of the samples to the standard curve.

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