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Drug Names
Generic Name: Human Herpesvirus 8 (HHV-8) ELISA Kit
Purpose
Our Human Herpesvirus 8 (HHV-8) ELISA kit is for the qualitative determination of HHV-8 in Human serum, plasma, culture media or any biological fluid.
Principle
The ELISA is based on the the qualitative enzyme immunoassay technique.The Microplate provided in this kit has been pre-coated with an Antibody specific to HHV-8, make it to solid-phase antibody.Samples are added to the Microplate wells and combined to the specific antibody. Then a Horseradish Peroxidase (HRP)-conjugated antibody specific for HHV-8 is added to each Microplate well and incubated,so the antibody-antigen-Enzyme labeled antibody complex is formed.Following a wash to remove any unbound reagent,then the TMB substrate solution is added to each well. Only those wells that contain HHV-8 and HRP conjugated HHV-8 antibody will appear blue in color and then turn yellow after the addition of the stop solution. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm. The qualitative determination of HHV-8 is determined by comparing with the CUTOFF value.
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