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Drug Names
Generic Name: Human S100 calcium-binding protein A8/A9 complex (S100A8/A9) ELISA Kit
Purpose
Our Human S100 calcium-binding protein A8/A9 complex (S100A8/A9) ELISA kit is to assay S100A8/A9 levels in Human serum, plasma, culture media or any biological fluid.
Principle
This ELISA kit uses Sandwich-ELISA as the method. The Microelisa stripplate provided in this kit has been pre-coated with an antibody specific to S100A8/A9. Standards or samples are added to the appropriate Microelisa stripplate wells and combined to the specific antibody. Then a Horseradish Peroxidase (HRP)-conjugated antibody specific for S100A8/A9 is added to each Microelisa stripplate well and incubated. Free components are washed away. The TMB substrate solution is added to each well. Only those wells that contain S100A8/A9 and HRP conjugated S100A8/A9 antibody will appear blue in color and then turn yellow after the addition of the stop solution. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm. The OD value is proportional to the concentration of S100A8/A9. You can calculate the concentration of S100A8/A9 in the samples by comparing the OD of the samples to the standard curve.
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