Purpose

Our Human papillomavirus 11（HPV11） ELISA kit is to assay HPV11  levels in Human serum, plasma, culture media or any biological fluid.

 

Principle

This ELISA kit uses Sandwich-ELISA as the method. The Microelisa strip plate provided in this kit has been pre-coated with an antibody specific to HPV11. Standards or samples are added to the appropriate Microelisa strip plate wells and combined to the specific antibody. Then a Horseradish Peroxidase (HRP)-conjugated antibody specific for HPV11 is added to each Microelisa stripplate well and incubated. Free components are washed away. The TMB substrate solution is added to each well. Only those wells that contain HPV11 and HRP conjugated HPV11antibody will appear blue in color and then turn yellow after the addition of the stop solution. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm. The presence of HPV11 is determined by comparing with the CUTOFF value.

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