Drug Names

Generic Name：Human tumor marker DR-70(DR70) ELISA Kit

Purpose

Our Human tumor marker DR-70(DR70) ELISA kit is to assay DR70 levels in Human serum, plasma, culture media or any biological fluid.

Principle

This ELISA kit uses Sandwich-ELISA as the method. The Microelisa stripplate provided in this kit has been pre-coated with an antibody specific to DR70. Standards or samples are added to the appropriate Microelisa stripplate wells and combined to the specific antibody. Then a Horseradish Peroxidase (HRP)- conjugated antibody specific for DR70 is added to each Microelisa stripplate well and incubated. Free components are washed away. The TMB substrate solution is added to each well. Only those wells that contain DR70 and HRP conjugated DR70 antibody will appear blue in color and then turn yellow after the addition of the stop solution. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm. The OD value is proportional to the concentration of DR70. You can calculate the concentration of DR70 in the samples by comparing the OD of the samples to the standard curve.

 

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