Drug Names

Generic Name：Human Foot and Mouth Disease Virus type Asia I  antibody(FMDV-Asia I-Ab) ELISA Kit

Purpose

Our Human Foot and Mouth Disease Virus type Asia I  antibody(FMDV-Asia I-Ab)  ELISA kit is to assay FMDV-Asia I-Ab levels in Human  serum, plasma, culture media or any biological fluid.

Principle

This ELISA kit uses Sandwich-ELISA as the method. The Microelisa stripplate provided in this kit has been pre-coated with an antigen specific to FMDV-Asia I-Ab. Standards or samples are added to the appropriate Microelisa stripplate wells and combined to the specific antigen. Then a Horseradish Peroxidase (HRP)-conjugated antigen specific for FMDV-Asia I is added to each Microelisa stripplate well and incubated. Free components are washed away. The TMB substrate solution is added to each well. Only those wells that contain FMDV-Asia I-Ab and HRP conjugated FMDV-Asia I antigen will appear blue in color and then turn yellow after the addition of the stop solution. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm. The presence ofFMDV-Asia I-Ab is determined by comparing with the CUTOFF value.

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