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Purpose
This kit allows for the determination of EBV concentrations in Human serum, blood plasma, and other biological fluids.
Principle
This ELISA kit uses Sandwich-ELISA as the method. The Microelisa stripplate provided in this kit has been pre-coated with an antibody specific to Epstren barr virus (EBV). Standards or samples are added to the appropriate Microelisa stripplate wells and combined to the specific antibody. Then a Horseradish Peroxidase (HRP)-conjugated antibody specific for Epstren barr virus (EBV) is added to each Microelisa stripplate well and incubated. Free components are washed away. The TMB substrate solution is added to each well. Only those wells that contain Epstren barr virus (EBV) and HRP conjugated Epstren barr virus (EBV) antibody will appear blue in color and then turn yellow after the addition of the stop solution. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm. The presence of Epstren barr virus (EBV) is determined by comparing with the CUTOFF value.
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