Drug Names

Generic Name：Human Anti-Ganglioside M3 Antibody (GM3-Ab) ELISA Kit

Purpose

Our human Anti-Ganglioside M3 Antibody (GM3-Ab) ELISA kit is to assay GM3-Ab levels in Human serum, plasma, culture media or any biological fluid.

Principle

This ELISA kit uses Sandwich-ELISA as the method. The Microelisa stripplate provided in this kit has been pre-coated with an antigen specific to GM3-Ab. Standards or samples are added to the appropriate Microelisa stripplate wells and combined to the specific antigen. Then a Horseradish Peroxidase (HRP)-conjugated antigen specific for GM3  is added to each Microelisa stripplate well and incubated. Free components are washed away. The TMB substrate solution is added to each well. Only those wells that contain GM3-Aband HRP conjugated GM3 antigen will appear blue in color and then turn yellow after the addition of the stop solution. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm. The presence of GM3-Ab  is determined by comparing with the CUTOFF value.

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