Purpose

Our Human hepatitis C virus core (HCV -core)  ELISA kit is to assay HCV -corelevels in Human serum, plasma, culture media or any biological fluid.

 

Principle

This ELISA kit uses Sandwich-ELISA as the method. The Microelisa stripplate provided in this kit has been pre-coated with an antibody specific to  HCV -core  . Standards or samples are added to the appropriate Microelisa stripplate wells and combined to the specific antibody. Then a Horseradish Peroxidase (HRP)-conjugated antibody specific for HCV -core   is added to each Microelisa stripplate well and incubated. Free components are washed away. The TMB substrate solution is added to each well. Only those wells that contain HCV -core and HRP conjugated  HCV -core antibody will appear blue in color and then turn yellow after the addition of the stop solution. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm. The presence of HCV -core  is determined by comparing with the CUTOFF value.

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