Purpose

Our Human Anti-Toxocara canis IgE（Toxocara- IgE） ELISA kit is to assay Toxocara- IgE levels in Human serum, plasma, culture media or any biological fluid.

Principle

This ELISA kit uses Sandwich-ELISA as the method. The Microelisa stripplate provided in this kit has been pre-coated with an antigen specific to Toxocara- IgE. Standards or samples are added to the appropriate Microelisa stripplate wells and combined to the specific antigen. Then a Horseradish Peroxidase (HRP)-conjugated antigen specific for Toxocara is added to each Microelisa stripplate well and incubated. Free components are washed away. The TMB substrate solution is added to each well. Only those wells that contain Toxocara- IgE and HRP conjugated Toxocara antigen will appear blue in color and then turn yellow after the addition of the stop solution. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm. The presence of  Toxocara- IgE  is determined by comparing with the CUTOFF value.

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