Purpose

Our Human salmonella typhi antibody IgM (ST-IgM)  ELISA kit is to assay salmonella typhi  antibody IgM (ST-IgM) levels in Human serum, plasma, culture media or any biological fluid.

Principle

This ELISA kit uses Sandwich-ELISA as the method. The Microelisa stripplate provided in this kit has been pre-coated with an antigen specific to salmonella typhi  antibody IgM (ST-IgM) . Standards or samples are added to the appropriate Microelisa stripplate wells and combined to the specific antigen. Then a Horseradish Peroxidase (HRP)-conjugated antigen specific for salmonella typhi (ST)  is added to each Microelisa stripplate well and incubated. Free components are washed away. The TMB substrate solution is added to each well. Only those wells that contain salmonella typhi  antibody IgM (ST-IgM)  and HRP conjugated salmonella typhi (ST)  antigen will appear blue in color and then turn yellow after the addition of the stop solution. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm. The presence of  salmonella typhi  antibody IgM (ST-IgM)   is determined by comparing with the CUTOFF value.

 

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