Purpose

Our Human 20-Hydroxyeicosatetraenoic Acid (20-HETE) ELISA Kit is to assay Permeability Glycoprotein (P-gp)levels in Human serum, plasma, culture media or any biological fluid.

 

Principle

This ELISA kit uses Sandwich-ELISA as the method. The Microelisa stripplate provided in this kit has been pre-coated with an antibody specific to 20-HETE . Standards or samples are added to the appropriate Microelisa stripplate wells and combined to the specific antibody. Then a Horseradish Peroxidase (HRP)- conjugated antibody specific for 20-HETE is added to each Microelisa stripplate well and incubated. Free components are washed away. The TMB substrate solution is added to each well. Only those wells that contain c-Jun and HRP conjugated 12-HETE antibody will appear blue in color and then turn yellow after the addition of the stop solution. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm. The OD value is proportional to the concentration of 20-HETE  You can calculate the concentration of  20-HETE  the samples by comparing the OD of the samples to the standard curve.

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