Drug Names

Generic Name：Human Cytochrome P450 19A1(CYP19A1) ELISA Kit

Purpose

Our Human Cytochrome P450 19A1(CYP19A1) ELISA kit is to assay CYP19A1 levels

in Human serum, plasma, culture media or any biological fluid. Principle

This ELISA kit uses Sandwich-ELISA as the method. The Microelisa stripplate provided

in this kit has been pre-coated with an antibody specific to CYP19A1. Standards or samples

are added to the appropriate Microelisa stripplate wells and combined to the specific antibody. Then a Horseradish Peroxidase (HRP)- conjugated antibody specific for CYP19A1 is added

to each Microelisa stripplate well and incubated. Free components are washed away. The

TMB substrate solution is added to each well. Only those wells that contain CYP19A1 and

HRP conjugated CYP19A1 antibody will appear blue in color and then turn yellow after the

addition of the stop solution. The optical density (OD) is measured spectrophotometrically at

a wavelength of 450 nm. The OD value is proportional to the concentration of CYP19A1. You can calculate the concentration of CYP19A1 in the samples by comparing the OD of the

samples to the standard curve.

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