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Drug Names
Generic Name:Human Lysine Specific Demethylase 6A (KDM6A)ELISA Kit
Purpose
Our Human Lysine Specific Demethylase 6A (KDM6A)ELISA kit is to assay KDM6A levels in Human serum, plasma, culture media or any biological fluid.
Principle
This ELISA kit uses Sandwich-ELISA as the method. The Microelisa stripplate provided in this kit has been pre-coated with an antibody specific to KDM6A. Standards or samples are added to the appropriate Microelisa stripplate wells and combined to the specific antibody. Then a Horseradish Peroxidase (HRP)- conjugated antibody specific for KDM6A is added to each Microelisa stripplate well and incubated. Free components are washed away. The TMB substrate solution is added to each well. Only those wells that contain KDM6A and HRP conjugated KDM6Aantibody will appear blue in color and then turn yellow after the addition of the stop solution. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm. The OD value is proportional to the concentration of KDM6A. You can calculate the concentration of KDM6A in the samples by comparing the OD of the samples to the standard curve.
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