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Drug Names
Generic Name:Human Aldo-keto reductase family 1 member C4, AKR1C4 ELISA Kit
Purpose
Our Human Aldo-keto reductase family 1 member C4, AKR1C4 ELISA Kit is to assay AKR1C4 levels in serum, plasma, culture media or any biological fluid.
Principle
This ELISA kit uses Sandwich-ELISA as the method. The Microelisa stripplate provided in this kit has been pre-coated with an antibody specific to AKR1C4. Standards or samples are added to the appropriate Microelisa stripplate wells and combined to the specific antibody. Then a Horseradish Peroxidase (HRP)- conjugated antibody specific forAKR1C4 is added to each Microelisa stripplate well and incubated. Free components are washed away. The TMB substrate solution is added to each well. Only those wells that contain AKR1C4and HRP conjugated AKR1C4 antibody will appear blue in color and then turn yellow after the addition of the stop solution. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm. The OD value is proportional to the concentration of AKR1C4. You can calculate the concentration of AKR1C4 in the samples by comparing the OD of the samples to the standard curve.
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