Drug Names

Generic Name：Human Anti-Circumsporozoite Protein IgG ELISA Kit

 

Purpose

Our Human Anti-Circumsporozoite Protein IgG ELISA Kit is to assay Anti-Circumsporozoite IgG levels in serum, plasma, culture media or any biological fluid.

Principle

This ELISA kit uses Sandwich-ELISA as the method. The Microelisa stripplate provided in this kit has been pre-coated with an antibody specific to Anti-Circumsporozoite IgG. Standards or samples are added to the appropriate Microelisa stripplate wells and combined to the specific antibody. Then a Horseradish Peroxidase (HRP)-conjugated antibody specific for Anti-Circumsporozoite IgG is added to each Microelisa stripplate well and incubated. Free components are washed away. The TMB substrate solution is added to each well. Only those wells that contain Anti-Circumsporozoite IgG and HRP conjugated Anti-Circumsporozoite IgG antibody will appear blue in color and then turn yellow after the addition of the stop solution. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm. The OD value is proportional to the concentration of Anti-Circumsporozoite IgG. You can calculate the concentration of Anti-Circumsporozoite IgG in the samples by comparing the OD of the samples to the standard curve.

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