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Drug Names
Generic Name:Human Tumor necrosis factor α (TNF-α) ELISA Kit
Purpose
Our human tumor necrosis factor α (TNF-α) ELISA kit is to assay TNF-α levels in saliva or any biological fluid.
Principle
This ELISA kit uses Sandwich-ELISA as the method. The Microelisa stripplate provided in this kit has been pre-coated with an antibody specific to TNF-α. Standards or samples are added to the appropriate Microelisa stripplate wells and combined to the specific antibody. Then a Horseradish Peroxidase (HRP)- conjugated antibody specific for TNF-α is added to each Microelisa stripplate well and incubated. Free components are washed away. The TMB substrate solution is added to each well. Only those wells that contain TNF-α and HRP conjugated TNF-α antibody will appear blue in color and then turn yellow after the addition of the stop solution. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm. The OD value is proportional to the concentration of TNF-α. You can calculate the concentration of TNF-α in the samples by comparing the OD of the samples to the standard curve.
Username | Quantity | bought time |
Ha*** | 2 | 2023-06-02 |
Ed*** | 2 | 2023-04-07 |
Ia*** | 1 | 2021-10-23 |
Is*** | 2 | 2021-05-20 |
Na*** | 1 | 2020-09-21 |
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