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vDrug Names
Generic Name:Human Rhinovirus IgM ELISA Kit
Purpose
Our human Rhinovirus IgM ELISA kit is to assay Rhinovirus IgM levels in Human serum, plasma, culture media or any biological fluid.
Principle
This ELISA kit uses Sandwich-ELISA as the method. The Microelisa stripplate provided in this kit has been pre-coated with an antigen specific toRhinovirus IgM. Standards or samples are added to the appropriate Microelisa stripplate wells and combined to the specific antigen. Then a Horseradish Peroxidase (HRP)-conjugated antigen specific for Rhinovirus is added to each Microelisa stripplate well and incubated. Free components are washed away. The TMB substrate solution is added to each well. Only those wells that contain Rhinovirus IgM and HRP conjugated Rhinovirus antigen will appear blue in color and then turn yellow after the addition of the stop solution. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm. The presence of Rhinovirus IgM is determined by comparing with the CUTOFF value.
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