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Drug Names
Generic Name:Human Influenza A IgM ELISA Kit
Purpose
Our human Influenza A IgM ELISA kit is to assay Influenza A IgM levels in Human serum, plasma, culture media or any biological fluid.
Principle
This ELISA kit uses Sandwich-ELISA as the method. The Microelisa stripplate provided in this kit has been pre-coated with an antigen specific toInfluenza A IgM. Standards or samples are added to the appropriate Microelisa stripplate wells and combined to the specific antigen. Then a Horseradish Peroxidase (HRP)-conjugated antigen specific for Influenza A is added to each Microelisa stripplate well and incubated. Free components are washed away. The TMB substrate solution is added to each well. Only those wells that contain Influenza A IgM and HRP conjugated Influenza A antigen will appear blue in color and then turn yellow after the addition of the stop solution. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm. The presence of Influenza A IgM is determined by comparing with the CUTOFF value.
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