TEL: +86 571 56623320 EMAIL: [email protected]
Drug Names
Generic Name:Human Soluble alpha-Klotho (S-KLα)ELISA Kit
Purpose
Our Human Soluble alpha-Klotho (S-KLα)ELISA kit is to assay soluble S-KLαlevels in Human serum, plasma, culture media or any biological fluid.
Principle
This ELISA kit uses Sandwich-ELISA as the method. The Microelisa stripplate provided in this kit has been pre-coated with an antibody specific to S-KLα Standards or samples are added to the appropriate Microelisa stripplate wells and combined to the specific antibody. Then a Horseradish Peroxidase (HRP)- conjugated antibody specific for S-KLα is added to each Microelisa stripplate well and incubated. Free components are washed away. The TMB substrate solution is added to each well. Only those wells that contain S-KLα and HRP conjugated S-KLα antibody will appear blue in color and then turn yellow after the addition of the stop solution. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm. The OD value is proportional to the concentration of S-KLα. You can calculate the concentration of S-KLα in the samples by comparing the OD of the samples to the standard curve.
Scan Wechat Qrcode