TEL: +86 571 56623320 EMAIL: [email protected]
Drug Names
Generic Name:Human anti-endothelial cell antibody(AECA ) ELISA Kit
Purpose
Our Human anti-endothelial cell antibody(AECA ) ELISA kit is to assay AECA levels in serum, plasma, culture media or any biological fluid.
Principle
This ELISA kit uses Sandwich-ELISA as the method. The Microelisa stripplate provided in this kit has been pre-coated with an antigen specific to AECA. Standards or samples are added to the appropriate Microelisa stripplate wells and combined to the specific antigen. Then a Horseradish Peroxidase (HRP)- conjugated antigen specific for AECA is added to each Microelisa stripplate well and incubated. Free components are washed away. The TMB substrate solution is added to each well. Only those wells that contain AECA and HRP conjugated AECA antigen will appear blue in color and then turn yellow after the addition of the stop solution. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm. The OD value is proportional to the concentration of AECA. You can calculate the concentration of AECA in the samples by comparing the OD of the samples to the standard curve.
Scan Wechat Qrcode