Drug Names

Generic Name：Human coagulation factor Ⅻ activated ,FⅫa ELISA Kit

 

Purpose

Our Human coagulation factor Ⅻ activated ,FⅫa ELISA Kit is to assay FⅫa Enzyme Active in Human serum, plasma, culture media or any biological fluid.

Principle

This ELISA kit uses Sandwich-ELISA as the method. The Microelisa stripplate provided in this kit has been pre-coated with an antibody specific to FⅫa. Standards or samples are added to the appropriate Microelisa stripplate wells and combined to the specific antibody. Then a Horseradish Peroxidase (HRP)- conjugated antibody specific for FⅫa is added to each Microelisa stripplate well and incubated. Free components are washed away. The TMB substrate solution is added to each well. Only those wells that contain FⅫa and HRP conjugated FⅫa antibody will appear blue in color and then turn yellow after the addition of the stop solution. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm. The OD value is proportional to the concentration of FⅫa. You can calculate the concentration of FⅫa in the samples by comparing the OD of the samples to the standard curve.

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