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Drug Names
Generic Name:Human Endometrium Antibody (EMAb) ELISA Kit
Purpose
Our Human Endometrium Antibody (EMAb) ELISA kit is to assay EMAb levels in Human serum, plasma, culture media or any biological fluid.
Principle
This ELISA kit uses Sandwich-ELISA as the method. The Microelisa stripplate provided in this kit has been pre-coated with an antigen specific to EMAb. Standards or samples are added to the appropriate Microelisa stripplate wells and combined to the specific antigen. Then a Horseradish Peroxidase (HRP)-conjugated antigen specific for EMAb is added to each Microelisa stripplate well and incubated. Free components are washed away. The TMB substrate solution is added to each well. Only those wells that contain EMAb and HRP conjugated EM antigen will appear blue in color and then turn yellow after the addition of the stop solution. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm. The OD value is proportional to the concentration of EMAb. You can calculate the concentration of EMAb in the samples by comparing the OD of the samples to the standard curve.
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