TEL: +86 571 56623320 EMAIL: [email protected]
Drug Names
Generic Name:Human anti-cardiolipin antibody IgA/G/M (ACA-IgA/G/M)
ELISA Kit
Purpose
Our Human anti-cardiolipin antibody IgA/G/M, Antibody(ACA-IgA/G/M) ELISA kit is to for the qualitative determination of ACA-IgA/G/M in Human serum, plasma, culture media or any biological fluid.
Principle
The ELISA is based on the the qualitative enzyme immunoassay technique.The Microplate provided in this kit has been pre-coated with an antigen specific to ACA-IgA/G/M, make it to solid-phase antigen.Samples are added to the Microplate wells and combined to the specific antigen. Then a Horseradish Peroxidase (HRP)-conjugated antigen specific for ACA-IgA/G/M is added to each Microplate well and incubated,so the antigen-antibody-Enzyme labeled antigen complex is formed.Following a wash to remove any unbound reagent,then the TMB substrate solution is added to each well. Only those wells that contain ACA-IgA/G/M and HRP conjugated ACA antigen will appear blue in color and then turn yellow after the addition of the stop solution. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm. The qualitative determination of ACA-IgA/G/M is determined by comparing with the CUTOFF value.
Scan Wechat Qrcode