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Drug Names
Generic Name:Human Mumps IgG ELISA Kit (quantitative)
Purpose
Our Human Mumps IgG ELISA Kit is to assay Mumps IgG levels in Human serum, plasma, culture media or any biological fluid.
Principle
This ELISA kit uses Sandwich-ELISA as the method. The Microelisa stripplate provided in this kit has been pre-coated with an antigen specific to Mumps IgG. Standards or samples are added to the appropriate Microelisa stripplate wells and combined to the specific antigen. Then a Horseradish Peroxidase (HRP)- conjugated antigen specific for Mumps IgGis added to each Microelisa stripplate well and incubated. Free components are washed away. The TMB substrate solution is added to each well. Only those wells that contain Mumps IgG and HRP conjugated Mumps IgG antigen will appear blue in color and then turn yellow after the addition of the stop solution. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm. The OD value is proportional to the concentration of Mumps IgG. You can calculate the concentration of Mumps IgG in the samples by comparing the OD of the samples to the standard curve.
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