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Drug Names
Generic Name: Human Aldose reductase (AKR1B1) ELISA Kit (Enzyme Active)
Purpose
Our Human Aldose reductase (AKR1B1) ELISA Kit (Enzyme Active) is to assay AKR1B1 Enzyme Active in Human serum, plasma, culture media or any biological fluid.
Principle
This ELISA kit uses Sandwich-ELISA as the method. The Microelisa stripplate provided in this kit has been pre-coated with an antibody specific to AKR1B1 Standards or samples are added to the appropriate Microelisa stripplate wells and combined to the specific antibody. Then a Horseradish Peroxidase (HRP)-conjugated antibody specific for AKR1B1 is added to each Microelisa stripplate well and incubated. Free components are washed away. The TMB substrate solution is added to each well. Only those wells that contain AKR1B1 and HRP conjugated AKR1B1 antibody will appear blue in color and then turn yellow after the addition of the stop solution. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm. The OD value is proportional to the concentration of AKR1B1 You can calculate the concentration of AKR1B1 in the samples by comparing the OD of the samples to the standard curve.
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