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Drug Names
Generic Name:Human RNA polymerase III (Pol III) antibody ELISA Kit
Purpose
Our Human RNA polymerase III (Pol III) antibody ELISA kit is to assay Pol III levels in Human serum, plasma, culture media or any biological fluid.
Principle
This ELISA kit uses Sandwich-ELISA as the method. The Microelisa stripplate provided in this kit has been pre-coated with an antigen specific to Pol III. Standards or samples are added to the appropriate Microelisa stripplate wells and combined to the specific antigen. Then a Horseradish Peroxidase (HRP)-conjugated antigen specific for Pol III is added to each Microelisa stripplate well and incubated. Free components are washed away. The TMB substrate solution is added to each well. Only those wells that contain Pol III and HRP conjugated Pol III antigen will appear blue in color and then turn yellow after the addition of the stop solution. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm. The OD value is proportional to the concentration of Pol III. You can calculate the concentration of Pol III in the samples by comparing the OD of the samples to the standard curve.
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