TEL: +86 571 56623320 EMAIL: [email protected]
Kit Name
Generic Name:
Human Cluster Of Differentiation 33( CD33 ) ELISA Kit
Purpose
Human Cluster Of Differentiation 33( CD33 ) ELISA Kit to assay CD33 levels in Human serum, plasma, culture media or any biological fluid.
Principle
This ELISA kit uses Sandwich-ELISA as the method. The Microelisa stripplate provided in this kit has been pre-coated with an antibody specific to CD33. Standards or samples are added to the appropriate Microelisa stripplate wells and combined to the specific antibody. Then a Horseradish Peroxidase (HRP)- conjugated antibody specific for CD33 is added to each Microelisa stripplate well and incubated. Free components are washed away. The TMB substrate solution is added to each well. Only those wells that contain CD33 and HRP conjugated CD33 antibody will appear blue in color and then turn yellow after the addition of the stop solution. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm. The OD value is proportional to the concentration of CD33. You can calculate the concentration of CD33 in the samples by comparing the OD of the samples to the standard curve.
Scan Wechat Qrcode