TEL: +86 571 56623320 EMAIL: [email protected]
Drug Names
Generic Name: Human Chlamydia pneumoniae IgE,Cpn IgE ELISA Kit
Purpose
Our Human Chlamydia pneumoniae IgE, Cpn IgE ELISA Kit is to assay Cpn-IgE levels in Human serum, plasma, culture media or any biological fluid.
Principle
This ELISA kit uses Sandwich-ELISA as the method. The Microelisa stripplate provided in this kit has been pre-coated with an antigen specific to Cpn-IgE Standards or samples are added to the appropriate Microelisa stripplate wells and combined to the specific antigen. Then a Horseradish Peroxidase (HRP)-conjugated antigen specific for Cpn-IgE is added to each Microelisa stripplate well and incubated. Free components are washed away. The TMB substrate solution is added to each well. Only those wells that contain Cpn-IgE and HRP conjugated Cpn antigen will appear blue in color and then turn yellow after the addition of the stop solution. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm. The OD value is proportional to the concentration of Cpn-IgE You can calculate the concentration of Cpn-IgE in the samples by comparing the OD of the samples to the standard curve.
Scan Wechat Qrcode