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Drug Names
Generic Name:Human Mothers against decapentaplegic homolog 3 (Smad3) ELISA Kit
Purpose
Our Human Mothers Against Decapentaplegic Homolog 3 (Smad3) ELISA kit is to assay Smad3 levels in Human serum, plasma, culture media or any biological fluid.
Principle
This ELISA kit uses Sandwich-ELISA as the method. The Microelisa stripplate provided in this kit has been pre-coated with an antibody specific to Smad3, Standards or samples are added to the appropriate Microelisa stripplate wells and combined to the specific antibody. Then a Horseradish Peroxidase (HRP)-conjugated antibody specific for Smad3 is added to each Microelisa stripplate well and incubated. Free components are washed away. The TMB substrate solution is added to each well. Only those wells that contain Smad3 and HRP conjugated Smad3 antibody will appear blue in color and then turn yellow after the addition of the stop solution. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm. The OD value is proportional to the concentration of Smad3. You can calculate the concentration of Smad3 in the sample
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