ONLY FOR SCIENCE RESEARCH

Drug Names

Generic Name：Human anti-2019 nCoV(S) IgM ELISA Kit(Qualitative)

Principle

This kit was based on indirect enzyme-linked immune-sorbent assay technology. Recombinant 2019 nCoV spike protein (antigen) was pre-coated onto 96-well plates. The test samples were added to the wells, unbound conjugates were washed away with wash buffer. Then added HRP conjugated anti-human IgM, if there were any anti-2019-nCoV(S)-IgM in the samples, it would form a complex. TMB substrates were used to visualize HRP enzymatic reaction. It was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The optical density of developed color is read with a suitable photometer at 450nm with a selected reference wavelength within 650nm.

Materials provided with the kit

Item	Specifications(96T)	Storage
ELISA Microplate(Dismountable)	8×12	2-8°C
Negative Control (Ready-to-use)	1vial	2-8°C
Positive Control (Ready-to-use)	1vial	2-8°C
Sample Dilution Buffer	1vial	2-8°C
HRP-conjugated anti-human IgM antibody (Concentrated)	1vial	2-8°C
Antibody Dilution Buffer	1vial	2-8°C
Wash Buffer (25 x concentrate)	1vial	2-8°C
TMB Substrate	1vial	2-8°C
Stop solution	1vial	2-8°C
Plate Sealer	3pieces
Instruction manual	1copy

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