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Drug Names
Generic Name: Human Parvovirus B19, B19 ELISA Kit
Purpose
Our Human Parvovirus B19,B19ELISA kit is to for the qualitative determination of B19 in Human serum, plasma, culture media or any biological fluid.
Principle
The ELISA is based on the the qualitative enzyme immunoassay technique.The Microplate provided in this kit has been pre-coated with an Antibody specific to B19,make it to solid-phase antibody.Samples are added to the Microplate wells and combined to the specific antigen. Then a Horseradish Peroxidase (HRP)-conjugated antigen specific for B19 is added to each Microplate well and incubated,so the antibody-antigen-Enzyme labeled antibody complex is formed.Following a wash to remove any unbound reagent,then the TMB substrate solution is added to each well. Only those wells that contain B19 and HRP conjugated B19 antibody will appear blue in color and then turn yellow after the addition of the stop solution. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm. The qualitative determination of B19 is determined by comparing with the CUTOFF value.
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