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Drug Names
Generic Name:Rat Low Affinity Nerve Growth Factor Receptor (LNGFR) ELISA Kit
Purpose
Our Rat Low Affinity Nerve Growth Factor Receptor (LNGFR) ELISA kit is to assay LNGFR levels in Rat serum, plasma, culture media or any biological fluid.
Principle
This ELISA kit uses Sandwich-ELISA as the method. The Microelisa stripplate provided in this kit has been pre-coated with an antibody specific to LNGFR. Standards or samples are added to the appropriate Microelisa stripplate wells and combined to the specific antibody. Then a Horseradish Peroxidase (HRP)- conjugated antibody specific for LNGFR is added to each Microelisa stripplate well and incubated. Free components are washed away. The TMB substrate solution is added to each well. Only those wells that contain LNGFR and HRP conjugated LNGFR antibody will appear blue in color and then turn yellow after the addition of the stop solution. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm. The OD value is proportional to the concentration of LNGFR. You can calculate the concentration of LNGFR in the samples by comparing the OD of the samples to the standard curve.
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