Drug Names

Generic Name：Human Entamoeba Histolytica IgM, EH-IgM ELISA Kit

Purpose

Our Human Entamoeba Histolytica IgM, EH-IgM ELISA Kit is to assay EH-IgM levels in Human serum, plasma, culture media or any biological fluid.

Principle
This ELISA kit uses Sandwich-ELISA as the method. The Microelisa stripplate provided in this kit has been pre-coated with an antigen specific to EH-IgM. Standards or samples are added to the appropriate Microelisa stripplate wells and combined to the specific antigen. Then a Horseradish Peroxidase (HRP)-conjugated antigen specific for EH is added to each Microelisa stripplate well and incubated. Free components are washed away. The TMB substrate solution is added to each well. Only those wells that contain EH-IgM and HRP conjugated EH antigen will appear blue in color and then turn yellow after the addition of  the stop solution. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm. The OD value is proportional to the concentration of EH-IgM. You can calculate the concentration of EH-IgM in the samples by comparing the OD of the samples to the standard curve.

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