Drug Names

Generic Name: Human Protein Phosphatase 2 (PP2a) ELISA Kit (Enzyme activity)

Purpose

Our Human Protein Phosphatase 2 (PP2a) ELISA kit (Enzyme activity) is to assay PP2a Enzyme Active in Human serum, plasma, culture media or any biological fluid.

Principle

This ELISA kit uses Sandwich-ELISA as the method. The Microelisa stripplate provided in this kit has been pre-coated with an antibody specific to PP2a. Standards or samples are added to the appropriate Microelisa stripplate wells and combined to the specific antibody. Then a Horseradish Peroxidase (HRP)-conjugated antibody specific for PP2a is added to each Microelisa stripplate well and incubated. Free components are washed away. The TMB substrate solution is added to each well. Only those wells that contain PP2a and HRP conjugated PP2a antibody will appear blue in color and then turn yellow after the addition of the stop solution. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm. The OD value is proportional to the concentration of PP2a. You can calculate the concentration of PP2a in the samples by comparing the OD of the samples to the standard curve.

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