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Drug Names
Generic Name: Human Herpesvirus 8 Antibody IgG (HHV8-IgG) ELISA Kit (Quantitative)
Purpose
Our Human Herpesvirus 8 Antibody IgG (HHV8-IgG) ELISA kit (Quantitative) is to assay HHV8-IgG levels in Human serum, plasma, culture media or any biological fluid.
Principle
This ELISA kit uses Sandwich-ELISA as the method. The Microelisa stripplate provided in this kit has been pre-coated with an antigen specific to HHV8-IgG. Standards or samples are added to the appropriate Microelisa stripplate wells and combined to the specific antigen . Then a Horseradish Peroxidase (HRP)-conjugated antigen specific for HHV8 is added to each Microelisa stripplate well and incubated. Free components are washed away. The TMB substrate solution is added to each well. Only those wells that contain HHV8-IgG and HRP conjugated HHV8 antigen will appear blue in color and then turn yellow after the addition of the stop solution. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm. The OD value is proportional to the concentration of HHV8-IgG. You can calculate the concentration of HHV8-IgG in the samples by comparing the OD of the samples to the standard curve.
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